Chemical reactivity of RNA and its modifications with hydrazine.

ABSTRACT: RNA modifications are essential for the regulation of cellular processes and have a key role in diseases such as cancer and neurological disorders. A major challenge in the analysis of RNA modification is the differentiation between isomers, including methylated nucleosides as well as uridine and pseudouridine. A solution is their differential chemical reactivity which enables isomer discrimination by mass spectrometry (MS) or sequencing. In this study, we systematically determine the chemical reactivity of hydrazine with RNA and its native modifications in an aniline-free environment. We optimize the conditions to achieve nearly full conversion of all uridines while avoiding RNA cleavage. We apply the conditions to native tRNA^Phe which allows discrimination of pseudouridine and

SUBMITTER: Yesiltac-Tosun N 

PROVIDER: S-EPMC11829040 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature