Functional improvement of natural Saccharomyces cerevisiae yeast strains by cell surface molecular engineering.

ABSTRACT:

Background

Cellular boundaries of microorganisms can be modified by the expression in the cell wall of specific proteins endowed with relevant properties, improving their functional performance. So far, the surface display (SD) technique had been widely employed in the yeast Saccharomyces cerevisiae, but it was limited to few laboratory strains and never explored in sauvage strains, i.e., isolated from natural environment, which are featured by higher levels of genetic variability, leading to peculiar phenotypic traits of possible advantage in biotechnology.

Results

In this work, a series of plasmids performing SD in natural yeast strains have been generated and further characterized by multiple functional and biochemical assays, providing the first experimental evidence tha

SUBMITTER: Granuzzo S 

PROVIDER: S-EPMC11829369 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature