Overexpressed NEK2 contributes to progression and cisplatin resistance through activating the Wnt/β-catenin signaling pathway in cervical cancer.

ABSTRACT:

Background

Cervical cancer ranks as the fourth most common cancer among women, with cisplatin resistance posing a significant challenge to the long-term survival of patients.

Methods

The roles of NEK2 in cervical cancer were examined through bioinformatics analysis. Transfection efficiency and molecular mechanisms were assessed using real-time quantitative polymerase chain reaction (qRT-PCR) and western blotting (WB). To evaluate cell functions, a series of assays, including cell counting kit-8 (CCK-8), wound healing, transwell, colony formation, and flow cytometry (FCM), were performed on HeLa, SiHa, and HeLa/DDP (cisplatin-resistant) cell lines.

Results

We found that NEK2 is upregulated in cervical cancer tissues compared to normal tissues and is further elevated in

SUBMITTER: Haiye J 

PROVIDER: S-EPMC11829479 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature