Anti-citrullinated protein antibody detection by hemagglutination.

ABSTRACT:

Objectives

ACPAs play an important role in the classification of RA and can be detected by serological tests. Several ACPA detection assays are available for clinical use, which are all based on ELISA(-like) tests with citrullinated peptides (CCP2) or alternative citrullinated proteins/peptides. We aimed to facilitate ACPA detection in low-volume laboratories and resource-poor environments by developing a rapid and easy-to-perform test.

Methods

We designed and generated an agglutination mediator by protein engineering. A single-chain antibody fragment that binds to glycophorin A, one of the major surface proteins of erythrocytes, was conjugated to a CCP2-like peptide. This agglutination mediator was used to assess ACPA in RA and PsA patients and in healthy individuals.

Re

SUBMITTER: Kruis I 

PROVIDER: S-EPMC11829806 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature