Gene expression regulation and polyadenylation in ulcerative colitis via long-chain RNA sequencing.

ABSTRACT:

Background

Ulcerative colitis (UC) is an immune-mediated chronic intestinal disease, with a pathogenesis that remains incompletely understood. The purpose of this study is to analyze the difference of gene expression between UC patients and healthy controls using Oxford Nanopore Technology's long-read RNA sequencing (ONT-RNA-seq) and to explore how alternative polyadenylation (APA) site selection contributes to UC pathogenesis.

Methods

Colon tissue samples from UC and normal controls (NC) were collected, and total RNA was extracted and sequenced using ONT-RNA-seq technology. Various bioinformatics analyses were performed, including differential expression gene (DEG) analysis, functional enrichment analysis, APA site analysis, and prediction miRNAs and RNA binding proteins (R

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC11830181 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature