Urine proteomics-based analysis identifies CHI3L1 as an immune marker and potential therapeutic target for bladder cancer.

ABSTRACT:

Background

Bladder cancer (BCa) is a prevalent malignancy characterized by a poor prognosis. Numerous studies have increasingly recognized the role of M2 macrophages in cancer progression. Consequently, our objective is to investigate hub genes in BCa associated with M2 macrophages, assessing their prognostic significance and exploring potential regulatory mechanisms.

Methods

We performed a comprehensive bioinformatics analysis using data from urine proteomics, the Gene Expression Omnibus (GEO) database, and The Cancer Genome Atlas (TCGA) database, in conjunction with machine learning methods such as LASSO and SVM to identify intersections of differentially expressed genes (DEGs). Subsequently, the role of hub genes in BCa was validated in vitro and in vivo using CCK-8 assay

SUBMITTER: Wang C 

PROVIDER: S-EPMC11830209 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature