Protein identification using Cryo-EM and artificial intelligence guides improved sample purification.

ABSTRACT: Protein purification is essential in protein biochemistry, structural biology, and protein design, enabling the determination of protein structures, the study of biological mechanisms, and the characterization of both natural and de novo designed proteins. However, standard purification strategies often encounter challenges, such as unintended co-purification of contaminants alongside the target protein. This issue is particularly problematic for self-assembling protein nanomaterials, where unexpected geometries may reflect novel assembly states, cross-contamination, or native proteins originating from the expression host. Here, we used an automated structure-to-sequence pipeline to first identify an unknown co-purifying protein found in several purified designed protein samples. By integr

SUBMITTER: Carr KD 

PROVIDER: S-EPMC11830286 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature