Single-cell transcriptomes reveal cell-type-specific and sample-specific gene function in human cancer.

ABSTRACT: Accurate annotation of gene function in individual samples and even in each cell type is essential for understanding the pathogenesis of cancers. Single-cell RNA-sequencing (scRNA-seq) provides unprecedented resolution to decipher gene function. In order to explore how scRNA-seq contributes to the understanding of gene function in cancers, we constructed an assessment framework based on co-expression network and neighbor-voting method using 116,814 cells. Compared with bulk transcriptome, scRNA-seq recalled more experimentally verified gene functions. Surprisingly, scRNA-seq revealed cell-type-specific functions, especially in immune cells, whose expression profile recalled immune-related functions that were not discovered in cancer cells. Furthermore, scRNA-seq discovered sample-specific

SUBMITTER: Yuan H 

PROVIDER: S-EPMC11830296 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature