Assessment of liver graft quality during hypothermic oxygenated perfusion: The first international validation study.

ABSTRACT:

Background & aims

While it is currently assumed that liver assessment is only possible during normothermic machine perfusion, there is uncertainty regarding a reliable and quick prediction of graft injury during ex situ hypothermic oxygenated perfusion (HOPE). We therefore intended to test, in an international liver transplant cohort, recently described mitochondrial injury biomarkers measured during HOPE before liver transplantation.

Methods

Perfusate samples of human livers from ten centers in seven countries with HOPE experience were analyzed for released mitochondrial compounds, i.e. flavin mononucleotide (FMN), NADH, purine derivatives and inflammatory markers. Livers deemed unsuitable for transplantation served as negative controls.

Results

We collected 473 perf

SUBMITTER: Eden J 

PROVIDER: S-EPMC11830552 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature