Is There a Key Primer for Amplification of Core Land Plant DNA Barcode Regions (rbcL and matK)?

ABSTRACT: The DNA barcode is a technique for molecular identification of species. Two core genes, matK and rbcL, are widely used for land plants. In this technique, the selection of primers is a fundamental step for the success of amplification. Then, we aim to evaluate the primer amplification capability for the DNA barcode regions rbcL and matK. We extracted primer sequences from DNA barcode studies in the Web of Science and used chloroplast genome sequences from NCBI for in silico PCR tests using OpenprimeR. Physicochemical properties of in silico PCR were evaluated using OpenprimeR. Our literature review resulted in 366 and 489 different rbcL and matK primers. These were tested in 8665 sequences, 8463 species from 98 orders. Evaluating only

SUBMITTER: Corvalan LCJ 

PROVIDER: S-EPMC11830564 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature