Rapid and simple detection of <i>Candida albicans</i> using closed dumbbell-mediated isothermal amplification.

ABSTRACT:

Introduction

Candida albicans, a human fungal pathogen, multiplies to invade body cells and causes fungal diseases in the condition of insufficient body's immune function. Early detection of C. albicans is required to guide appropriate prevention and treatment.

Methods

The purpose of this study was to establish a C. albicans assay based on newly developed closed dumbbell-mediated isothermal amplification (CDA) to achieve rapid and simple point of care diagnostic. The CDA technique was carried out by specific primers targeting at the conserved C. albicans ITS2 gene. All primers were selected and evaluated by real-time fluorescence monitoring and endpoint visual judgement indicated by hydroxy naphthol blue (HNB). Optimal primers and accelerate prime

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11830661 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature