A supramolecular FRET signal amplification nanoprobe for high contrast and synchronous <i>in situ</i> imaging of cell surface receptor homodimers/heterodimers.

ABSTRACT: Epidermal growth factor receptor (EGFR) homodimers and heterodimers play significant roles in a variety of tumors, but current imaging probes remain problematic due to restricted contrast and sensitivity. Thus, we have developed aptamer-mediated activated conformational transitions to target the EGFR and HER2. Furthermore, based on signal amplification techniques, especially the FRET fluorescence enhancement properties of poly-β-CD, supramolecular FRET signal amplification nanoprobes were constructed to improve imaging contrast and sensitivity. The results confirmed that the fluorescence intensity of the supramolecular FRET group probe is 1.2 to 1.3 times that of the multi-FRET group and 11.3 to 23.2 times that of the single-FRET group. The results further confirmed that the supramolecular

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11831222 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature