A Versatile Drift-Free Super-Resolution Imaging Method via Oblique Bright-Field Correlation.

ABSTRACT: High-resolution optical microscopy, particularly super-resolution localization microscopy, requires precise real-time drift correction to maintain constant focus at nanoscale precision during the prolonged data acquisition. Existing methods, such as fiducial marker tracking, reflection monitoring, and bright-field image correlation, each provide certain advantages but are limited in their broad applicability. In this work, a versatile and robust drift correction technique is presented for single-molecule localization-based super-resolution microscopy. It is based on the displacement analysis of bright-field image features of the specimen with oblique illumination. By leveraging the monotonic relationship between the displacement of image features and axial positions, this method can precis

SUBMITTER: Ma H 

PROVIDER: S-EPMC11831467 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature