A Modular Engineered DNA Nanodevice for Precise Profiling of Telomerase RNA Location and Activity.

ABSTRACT: Increased telomerase activity has been considered as a conspicuous sign of human cancers. The catalytic cores of telomerase involve a reverse transcriptase and the human telomerase RNA (hTR). However, current detection of telomerase is largely limited to its activity at the tissue and single-cell levels. To reveal the precise distribution of subcellular hTR and telomerase activity, here a modular engineered DNA nanodevice (DNA-ND) is designed capable of imaging hTR and telomerase activity in cytoplasm and nucleus, enabling colocalization analysis. DNA-ND is a modular DNA complex comprising hTR and telomerase activity detection modules, which respectively sense intercellular hTR and telomerase activity via target-sensitive allosteric transition of DNA switches, actuating orthogonal activati

SUBMITTER: Zhang SY 

PROVIDER: S-EPMC11831533 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature