Nanopore sequencing enables novel detection of deuterium incorporation in DNA.

ABSTRACT: Identifying active microbes is crucial to understand their role in ecosystem functions. Metabolic labeling with heavy, non-radioactive isotopes, i.e., stable isotope probing (SIP), can track active microbes by detecting heavy isotope incorporation in biomolecules such as DNA. However, the detection of heavy isotope-labeled nucleotides directly during sequencing has, to date, not been achieved. In this study, Oxford nanopore sequencing was utilized to detect heavy isotopes incorporation in DNA molecules. Two isotopes widely used in SIP experiments were employed to label a bacterial isolate: deuterium (D, as D₂O) and carbon-13 (¹³C, as glucose). We hypothesize that labeled DNA is distinguishable from unlabeled DNA by changes in the nanopore signal. To verify this distin

SUBMITTER: Honer Zu Siederdissen C 

PROVIDER: S-EPMC11832021 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature