De novo identification of universal cell mechanics gene signatures.

ABSTRACT: Cell mechanical properties determine many physiological functions, such as cell fate specification, migration, or circulation through vasculature. Identifying factors that govern the mechanical properties is therefore a subject of great interest. Here, we present a mechanomics approach for establishing links between single-cell mechanical phenotype changes and the genes involved in driving them. We combine mechanical characterization of cells across a variety of mouse and human systems with machine learning-based discriminative network analysis of associated transcriptomic profiles to infer a conserved network module of five genes with putative roles in cell mechanics regulation. We validate in silico that the identified gene markers are universal, trustworthy, and specific to the mechanic

SUBMITTER: Urbanska M 

PROVIDER: S-EPMC11832173 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature