Clearing the path for whole-mount labeling and quantification of neuron and vessel density in adipose tissue.

ABSTRACT: White adipose tissue (WAT) comprises a plethora of cell types beyond adipocytes forming a regulatory network that ensures systemic energy homeostasis. Intertissue communication is facilitated by metabolites and signaling molecules that are spread by vasculature and nerves. Previous works have indicated that WAT responds to environmental cues by adapting the abundance of these 'communication routes'; however, the high intra-tissue heterogeneity questions the informative value of bulk or single-cell analyses and underscores the necessity of whole-mount imaging. The applicability of whole-mount WAT-imaging is currently limited by two factors - (1) methanol-based tissue clearing protocols restrict the usable antibody portfolio to methanol-resistant antibodies and (2) the vast amounts of data r

SUBMITTER: Rauchenwald T 

PROVIDER: S-EPMC11832183 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature