Diverse vaginal microbiota in healthy Japanese women: a combined relative and quantitative analyses.

ABSTRACT:

Introduction

This cross-sectional study aimed to characterize the viable vaginal microbiota and identify host factors influencing this microbiota by employing a combination of relative and quantitative analyses.

Methods

Twenty-four vaginal fluid samples were collected from healthy adult Japanese women for analysis. Vaginal fluid pH was measured using a portable pH meter. DNA was extracted from the vaginal fluid, and the 16S ribosomal RNA gene sequences in the V3-V4 regions were analyzed to identify bacterial species. Additionally, the vaginal fluid was cultured on four types of selective agar plates. The predominant species in the growing colonies were identified using colony polymerase chain reaction, and the colonies were counted.

Results

The vaginal microbiota was

SUBMITTER: Ito M 

PROVIDER: S-EPMC11832463 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature