Immunoassay-mass spectrometry to identify <i>Brucella melitensis</i>.

ABSTRACT: Two factors frequently impede accurate bacterial identification using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): inadequate bacterial abundance in real samples and bacterial combinations. For MALDI-TOF MS analysis and libraries for bacterial identification, time-consuming culture procedures are necessary to achieve sufficient concentration and isolation of a single bacterium. When dealing with hazardous bacteria like Brucella, which are more difficult to handle and cure, this problem becomes even more crucial. To overcome these obstacles, Fe₃O₄ magnetic nanoparticles (MNPs) linked with Brucella-specific antibodies and MALDI-TOF MS analysis have been used to create a quick and accurate technique for direct

SUBMITTER: Sharif A 

PROVIDER: S-EPMC11832529 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature