¹⁵N-detected TROSY for ¹H-¹⁵N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality.

ABSTRACT: Intrinsically disordered proteins and protein regions are central to many biological processes but difficult to characterize at atomic resolution. Nuclear magnetic resonance is particularly well-suited for providing structural and dynamical information on intrinsically disordered proteins, but existing NMR methodologies need to be constantly refined to provide greater sensitivity and resolution, particularly to capitalise on the potential of high magnetic fields to investigate large proteins. In this paper, we describe how ¹⁵N-detected 2D NMR experiments can be optimised for better performance. We show that using selective aliphatic ¹H decoupling in N-TROSY type experiments results in significant increases in sensitivity and resolution for a prototypical intrinsically

SUBMITTER: Rodella MA 

PROVIDER: S-EPMC11832566 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature