Extracellular vesicles from pancreatic cancer and its tumour microenvironment promote increased Schwann cell migration.

ABSTRACT:

Background

Pancreatic ductal adenocarcinoma (PDAC) exhibits a high frequency of neural invasion (NI). Schwann cells (SCs) have been shown to be reprogrammed to facilitate cancer cell migration and invasion into nerves. Since extracellular vesicles (EVs) affect the tumour microenvironment and promote metastasis, the present study analysed the involvement of EVs from pancreatic cancer cells and their microenvironment in altering SC phenotype as part of the early events in the process of NI.

Methods

EVs were isolated from human/murine PDAC cells, pancreatic stellate cells (PSCs), human tissues and plasma to perform a novel 3D migration assay, qRT-PCR and western blot. Kaplan-Meier and Cox regression analyses were employed to evaluate the clinical potential of plasma EV-derived

SUBMITTER: Wong FC 

PROVIDER: S-EPMC11832759 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature