Identification of genes involved in cytochrome c biogenesis in Shewanella oneidensis, using a modified mariner transposon.

ABSTRACT: A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome c production.

SUBMITTER: Bouhenni R 

PROVIDER: S-EPMC1183303 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature