Sodium nitrite orchestrates macrophage mimicry of tongue squamous carcinoma cells to drive lymphatic metastasis.

ABSTRACT:

Background

Tongue squamous cell carcinoma (TSCC) is a malignant oral cancer with unclear pathogenesis that shows a tendency for early-stage lymphatic metastasis. This results in a poor prognosis, with a low 5-year survival rate. Dietary sodium nitrite (NaNO₂) has proposed associations with disease, including cancer. However, a direct relationship between NaNO₂ and TSCC has not been established.

Methods

In vitro and in vivo assays were used to investigate the role of NaNO₂ in TSCC. Protein expression in TSCC specimens was detected by immunohistochemistry and immunofluorescence. The molecular mechanism was determined using RT-qPCR, western blot, RNA-seq, luciferase reporter assays, migration assays, and FACS analysis. More detail of methods can

SUBMITTER: Lu X 

PROVIDER: S-EPMC11833070 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature