Nonlinear mixed-effects models to analyze actin dynamics in dendritic spines.

ABSTRACT: Fluorescence recovery after photobleaching (FRAP) allows to study actin-turnover in dendritic spines by providing recovery trajectories over time within a nested data structure (i.e. spine/neuron/culture). Statistical approaches to FRAP usually consider one-phase association models to estimate recovery-curve-specific parameters and test statistical hypotheses on curve parameters either at the spine or neuron level, ignoring the nested data structure. However, this approach leads to pseudoreplication concerns. We propose a nonlinear mixed-effects model to integrate the one-phase association model estimate with the nested data structure of FRAP experiments; this also allows us to model heteroscedasticity and time dependence in the data. We used this approach to evaluate the effect of the dow

SUBMITTER: Di Credico G 

PROVIDER: S-EPMC11833086 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature