<i>In vitro</i> gene editing using primary cells derived from Cas9-expressing pigs.

ABSTRACT: Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology has significantly facilitated the generation of gene-edited (GE) pigs. Although GE pigs are promising for agricultural and biomedical applications, the entire process of generating useful GE pigs is time- and labor-intensive. To overcome this, in vivo gene-editing techniques have been developed, where Cas9 nuclease and single guide RNA (sgRNA) are directly injected into animals; however, their efficiency remains low owing to the large size of the nuclease. In this study, we generated a Cas9-expressing pig by inserting the Cas9 gene into the ROSA26 locus, resulting in its constitutive expression in various tissues. We also confirmed the pig's fertility. In vitro exper

SUBMITTER: Kim S 

PROVIDER: S-EPMC11833195 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature