Single-cell RNA sequencing integrated with bulk RNA sequencing analysis of clock circadian regulator with prognostic and immune microenvironment in thyroid cancer.

ABSTRACT:

Background

Disruption of circadian rhythm was found to be associated with immune infiltration and thyroid cancer. However, the role of clock circadian regulator (CLOCK) in the progression of thyroid cancer and its immune microenvironment remains largely unexplored. Therefore, our aim was to explore the role and potential mechanism of CLOCK in thyroid cancer.

Methods

Single cell sequencing analysis and bulk RNA sequencing analysis was used for LASSO regression and Kaplan-Meier survival estimates. Potential mechanism analysis were gained through KEGG/GO analysis, GSEA analysis and PPI network. In vivo and in vitro experiment was used for further validation.

Results

The result showed CLOCK protein was overexpressed in thyroid cancer compared with normal tissue in both th

SUBMITTER: Shen Z 

PROVIDER: S-EPMC11833347 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature