Involvement of a branched-chain aminotransferase in production of volatile sulfur compounds in Yarrowia lipolytica.

ABSTRACT: The enzymatic degradation of L-methionine and the subsequent formation of volatile sulfur compounds (VSCs) are essential for the development of the typical flavor in cheese. In the yeast Yarrowia lipolytica, the degradation of L-methionine was accompanied by the formation of the transamination product 4-methylthio-2-oxobutyric acid. A branched-chain aminotransferase gene (YlBCA1) of Y. lipolytica was amplified, and the L-methionine-degrading activity and the aminotransferase activity were measured in a genetically modified strain and compared to those of the parental strain. Our work shows that L-methionine degradation via transamination is involved in formation of VSCs in Y. lipolytica.

SUBMITTER: Bondar DC 

PROVIDER: S-EPMC1183345 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature