Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides.

ABSTRACT: Animal venoms, distinguished by their unique structural features and potent bioactivities, represent a vast and relatively untapped reservoir of therapeutic molecules. However, limitations associated with comprehensively constructing and expressing highly complex venom and venom-like molecule libraries have precluded their therapeutic evaluation via high-throughput screening. Here, we developed an innovative computational approach to design a highly diverse library of animal venoms and "metavenoms". We used programmable M13 hyperphage display to preserve critical disulfide-bonded structures for highly parallelized single-round biopanning with quantitation via high-throughput DNA sequencing. Our approach led to the discovery of Kunitz-type domain containing proteins that target the human it

SUBMITTER: Hsiao MH 

PROVIDER: S-EPMC11833617 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature