Photoacoustic imaging of rat kidney tissue oxygenation using second near-infrared wavelengths.

ABSTRACT:

Significance

Conventionally, spectral photoacoustic imaging (sPAI) to assess tissue oxygenation ( sO2 ) uses optical wavelengths in the first near-infrared (NIR-I) window. This limits the maximum photoacoustic imaging depth due to the high spectral coloring of biological tissues and has been a major barrier to the clinical translation of the technique.

Aim

We demonstrate the second near-infrared (NIR-II) tissue optical window (950 to 1400 nm) for the assessment of blood and tissue sO2 .

Approach

The NIR-II PA spectra of oxygenated and deoxygenated hemoglobin were first characterized using a phantom. Optimal wavelengths to minimize spectral coloring were identified. The resulting NIR-II PA imaging methods were then validated in vivo by measuring kidney sO2 in ad

SUBMITTER: Vincely VD 

PROVIDER: S-EPMC11833698 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature