Electrochemically modulated single-molecule localization microscopy for <i>in vitro</i> imaging cytoskeletal protein structures.

ABSTRACT: A new concept of electrochemically modulated single-molecule localization super-resolution imaging is developed. Applications of single-molecule localization super-resolution microscopy have been limited due to insufficient availability of qualified fluorophores with favorable low duty cycles. The key for the new concept is that the "On" state of a redox-active fluorophore with unfavorable high duty cycle could be driven to "Off" state by electrochemical potential modulation and thus become available for single-molecule localization imaging. The new concept was carried out using redox-active cresyl violet with unfavorable high duty cycle as a model fluorophore by synchronizing electrochemical potential scanning with a single-molecule localization microscope. The two cytoskeletal protein st

SUBMITTER: Lei C 

PROVIDER: S-EPMC11834056 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature