An automated and high-throughput approach for enhanced precision of adenoviral titering.

ABSTRACT: Accurate quantification of viral vectors and vaccines is a crucial step required before any downstream use of virus preparations. The conventional immunocytochemistry-based method of adenovirus quantification has been widely used, but there are many areas for improvement toward accuracy and resource consumption savings to reduce viral miscalculation and wastage of vaccination materials. In this work, a one-step approach is implemented for optimized adenoviral quantification that uses a single antibody coupled with automated, high-throughput image acquisition and subsequent batch analysis. First, cells are infected with the adenovirus of interest and stained using the Hexon protein. Then, multichannel automated image acquisition via the Invitrogen EVOS M7000 Imaging System is performed. Las

SUBMITTER: Bottega P 

PROVIDER: S-EPMC11834061 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature