Constructing a 3D co-culture <i>in vitro</i> synovial tissue model for rheumatoid arthritis research.

ABSTRACT: The development and exploration of highly effective drugs for rheumatoid arthritis remains an urgent necessity. However, current disease research models are no longer sufficient to meet the rapid development of high-throughput drug screening. In this study, bacterial cellulose simulating the structure of extracellular matrix was used as a 3D culture platform, and THP-1-derived M1 macrophages, representing the inflammatory component, human umbilical vein endothelial cells (HUVECs), simulating the vascular component, and rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs), embodying the synovial pathology, were co-cultured to simulate the pathological microenvironment in RA synovial tissues, and synovial organoids were constructed. Under three-dimensional (3D) culture conditions, the

SUBMITTER: Wang X 

PROVIDER: S-EPMC11834117 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature