Regulation of co-translational mRNA decay by PAP and DXO1 in Arabidopsis.

ABSTRACT:

Background

mRNA decay is central in the regulation of mRNA homeostasis in the cell. The recent discovery of a co-translational mRNA decay pathway (also called CTRD) has changed our understanding of the mRNA decay process. This pathway has emerged as an evolutionarily conversed mechanism essential for specific physiological processes in eukaryotes, especially in plants. In Arabidopsis, this pathway is targeted mainly by the exoribonuclease XRN4. However, the details of the molecular regulation of this pathway are still unclear.

Results

In this study, we first tested the role of the 3'-phosphoadenosine 5'-phosphate (PAP), an inhibitor of exoribonucleases in the regulation of CTRD. Using 5'Pseq approach, we discovered that FRY1 inactivation impaired XRN4-CTRD activity. Based on

SUBMITTER: Carpentier MC 

PROVIDER: S-EPMC11834196 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature