Targeting the NLRP3 in macrophages contributes to senescence cell clearance in radiation-induced skin injury.

ABSTRACT:

Background

The persistent accumulation of senescence cells is one of the characteristics of radiation-induced skin injury (RISI), leading to fibrosis and impaired healing. However, the reasons why these senescence cells are resistant to clearance remain unclear.

Methods

The mouse RISI model was established using an X-ray generator, and a shield was used to cover all areas except the skin of the right leg or back for protecting surrounding tissue. ScRNA sequencing, immunohistochemistry, immunofluorescence, qPCR, western blot, primary cell co-culture system and fluorescence microsphere phagocytosis assay were performed for the functional and mechanistic investigations.

Results

The dynamic changes of senescence cell levels and multiple immune cell levels during RISI were

SUBMITTER: Liu G 

PROVIDER: S-EPMC11834210 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature