Performance Comparison of Liquid Chromatography and Paper Spray Ionization with Mass Spectrometry for Measuring Kinase Inhibitors in Human Plasma.

ABSTRACT: Kinase inhibitors are small-molecule drugs designed to target oncogenic mutations in cancer treatment. Although less toxic than conventional chemotherapy drugs, they can cause severe adverse effects in some patients, resulting in dose reduction and cessation. To evaluate if therapeutic drug monitoring of kinase inhibitors and their metabolites can improve toxicity assessment in patients, we developed and evaluated the analytical performance of two parallel methods utilizing liquid chromatography (LC) and paper spray (PS) ionization coupled with a triple quadrupole mass spectrometer (MS) for the measurement of dabrafenib, its major metabolite OH-dabrafenib, and trametinib in patient plasma samples. The PS-MS method yielded a faster sample analysis time (2 min) compared to the LC separation

SUBMITTER: Lahr RG 

PROVIDER: S-EPMC11834250 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature