Genomic re-sequencing reveals mutational divergence across genetically engineered strains of model archaea.

ABSTRACT: Archaeal molecular biology has been a topic of intense research in recent decades as their role in global ecosystems, nutrient cycles, and eukaryotic evolution comes to light. The hypersaline-adapted archaeal species Halobacterium salinarum and Haloferax volcanii serve as important model organisms for understanding archaeal genomics, genetics, and biochemistry, in part because efficient tools enable genetic manipulation. As a result, the number of strains in circulation among the haloarchaeal research community has increased in recent decades. However, the degree of genetic divergence and effects on genetic integrity resulting from the creation and inter-lab transfer of novel lab stock strains remain unclear. To address this, we performed whole-genome re-sequencing on a cross

SUBMITTER: Soborowski AL 

PROVIDER: S-EPMC11834397 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature