The interplay between <i>Acinetobacter baumannii</i> ZigA and SltB promotes zinc homeostasis and cell envelope integrity.

ABSTRACT: Acinetobacter baumannii is an opportunistic human pathogen that acquires nutrient metals from the vertebrate host amid infection. During zinc (Zn) scarcity, A. baumannii upregulates the expression of the predicted Zn metallochaperone, zigA. Loss of zigA compromises fitness during Zn deficiency, highlighting its role in this condition. To assess the contribution of ZigA to Zn-deficient A. baumannii, a multiparallel transposon sequencing and genetic interaction mapping approach was used. Transposon insertions in A1S_3027, encoding a predicted soluble lytic transglycosylase that tailors the bacterial cell wall, were enriched in the Zn-starved ΔzigA transposon library. Based on previous studies as well as structural and sequence homology, we des

SUBMITTER: Critchlow JM 

PROVIDER: S-EPMC11834433 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature