Evaluating small extracellular vesicle-based vaccination across heterologous <i>Salmonella</i> strains isolated from wastewater.

ABSTRACT: Salmonella infections pose significant public health challenges worldwide. The diversity of Salmonella strains, particularly those isolated from environmental and clinical sources, necessitates innovative approaches to prevention and treatment. Previous research has shown that small extracellular vesicles (sEVs) produced by macrophages during Salmonella Typhimurium infection can induce robust immune responses when used as a vaccine, offering complete protection in systemic infection models. In this study, we isolated 120 Salmonella strains from qPCR invA-positive wastewater samples collected in Gainesville, FL. These strains underwent enrichment, selection, and biochemical confirmation, followed by serotyping and whole genome sequencing. Two isolates,

SUBMITTER: Emerson LE 

PROVIDER: S-EPMC11834434 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature