Use of a mouse model for the isolation of Borrelia puertoricensis from soft ticks.

ABSTRACT: The isolation of tick-borne relapsing fever (TBRF) spirochetes has proven to be a useful tool to understand their distribution in geographic areas where the tick vectors inhabit. However, their isolation and culture are not easy and in general an animal model is needed to achieve this task. Here, argasid ticks were collected from a neighborhood in Ciudad Caucel, and they were identified as Ornithodoros (Alectorobius) puertoricensis. To determine whether these were infected with TBRF bacteria they were fed with healthy mice but only a low burden of spirochetes was observed. An immunosuppressed mouse model was used to feed the ticks suspected to be infected with spirochetes. After tick feeding, a higher number of bacteria was observed in blood samples, and spirochetes were successfully culti

SUBMITTER: Vazquez-Guerrero E 

PROVIDER: S-EPMC11835249 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature