Epithelial NSD2 maintains FMO-mediated taurine biosynthesis to prevent intestinal barrier disruption.

ABSTRACT:

Background

Inflammatory bowel disease (IBD) presents a significant challenge due to its intricate pathogenesis. NSD2, a histone methyltransferase responsible for dimethylating histone 3 at lysine 36, is associated with transcriptional activation. NSD2 expression is decreased in both the intestinal epithelial cells (IECs) of IBD patients and the IBD mouse model. However, the precise role of NSD2 in IBD remains unexplored.

Methods

Colon tissues from IBD mice, SW620 cells and MC38 cells, were used as research subjects. Clinical databases of IBD patients were analysed to investigate whether NSD2 expression is reduced in the occurrence of IBD. NSD2-knockout mice were generated to further investigate the role of NSD2 in IBD. The IECs were isolated for RNA sequencing and chromatin

SUBMITTER: Xu Y 

PROVIDER: S-EPMC11835373 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature