Evaluation of a PCR-based lateral flow device for detecting <i>Aspergillus</i> and <i>Candida</i> species from clinical specimens.

ABSTRACT:

Objectives

The diagnosis of invasive fungal diseases (IFDs) is time consuming and lacks sensitivity. In this research a rapid and easy to use immunochromatography-based DNA biosensor system was developed to detect Candida and Aspergillus pathogens at genus level, while specifically detecting Candida glabrata, Candida krusei and Aspergillus terreus. This system combines multiplex PCR with a lateral flow assay (LFA) dipstick.

Methods

Three separate multiplexed PCR reactions were designed together with a testing algorithm, using biotin, digoxigenin and Tamra fluorophore-labelled fungal internal transcribed spacer universal fungal primers, fungal genera-specific primers, and species-specific primers to produce labelled PCR products that were de

SUBMITTER: Khalid A 

PROVIDER: S-EPMC11835569 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature