Identification and functional analysis of Wall-Associated Kinase genes in Nicotiana tabacum.

ABSTRACT:

Introduction

Wall-associated kinases (WAKs) are pivotal in linking plant cell walls to intracellular signaling networks, thereby playing essential roles in plant growth, development, and stress responses.

Methods

The bioinformatics analysis was employed to identify WAK genes in tobacco. The expression levels of NtWAK genes were assessed by qRT-PCR. The subcellular localization of WAK proteins was observed in tobacco cells and Arabidopsis protoplasts. Kinase activity of the WAK proteins was evaluated through in vitro assays.

Results

We conducted a comprehensive genome-wide identification and analysis of the WAK gene family in tobacco (Nicotiana tabacum). A total of 44 WAK genes were identified in the tobacco genome, which were further

SUBMITTER: Li L 

PROVIDER: S-EPMC11835679 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature