Uncovering proteome variations and concomitant quality changes of different drying methods <i>Cordyceps sinensis</i> by 4D-DIA structural proteomics.

ABSTRACT:

Introduction

Cordyceps sinensis is a fungus, serves dual purposes as both a medicinal herb and a food source. Due to its high water content, fresh Cordyceps sinensis is difficult to preserve, necessitating the drying necessary to process Cordyceps sinensis.

Methods

Using 4D-DIA proteomics, researchers analyzed the proteome profiles of fresh Cordyceps sinensis (CK) under three different drying conditions: vacuum freeze-drying (FD), oven-drying (OD), and air-drying (AD). In addition, it was found that the protein and free sulfhydryl content of Cordyceps sinensis decreased significantly and the disulfide bond content increased after different drying methods.

Results and discussion

A total of 3762 proteins were identified, showing variat

SUBMITTER: Xiao M 

PROVIDER: S-EPMC11835701 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature