Exploring the cytotoxic effects of bioactive compounds from Alcea rosea against stem cell driven colon carcinogenesis.

ABSTRACT: Seven compounds were isolated from ethyl acetate extract of Alcea rosea and were examined for their cytotoxicity against HCT116, HT29 and SW480 colon cancer cells. It was found that two compounds (C4 and C5) exhibited strong anti-colon cancer activities. These two compounds were used to study their properties that include MTT activity (with IC50 of C4 as 74.71, 129.0 and 131.4 µg/ml in HCT116, HT29 and SW480 respectively, whereas IC50 of C5 as 128.1, 168.4 and 225.8 µg/ml in HCT116, HT29 and SW480 cells respectively), colony formation activity, wound healing activity, spheroid formation activity, DAPI-PI staining, acridine-orange and ethidium bromide staining, ROS measurement, and rhodamine-123 staining in both HCT116 and HT29 colon cancer cells. Both the compounds showed significant incre

SUBMITTER: Parry RA 

PROVIDER: S-EPMC11836269 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature