Metabolic profiling and transcriptome analysis of Sinomenium acutum provide insights into the biosynthesis of structurally diverse benzylisoquinoline alkaloids.

ABSTRACT: Sinomenium acutum, a traditional medicinal plant, has been utilized for millennia to alleviate various forms of rheumatic pain symptoms. The structurally diverse benzylisoquinoline alkaloids (BIAs) found in S. acutum are the primary contributors to its therapeutic efficacy, with sinomenine being the principal bioactive constituent. In this study, we employed an integrated transcriptomic and metabolomic approach to investigate BIA biosynthesis in S. acutum. Transcriptome sequencing, functional annotation, and differential gene expression analysis were combined with metabolite profiling to predict biosynthetic pathways of structurally diverse BIAs and screen candidate genes. Metabolomic analysis revealed significant stem-enriched accumulation of BIAs compared to leaves. Furthermore, we propo

SUBMITTER: Liu X 

PROVIDER: S-EPMC11836279 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature