High-sensitivity qPCR detection method based on silver flower-like LSPR-active material.

ABSTRACT: Quantitative polymerase chain reaction (qPCR) technology offers advantages such as easy operation and high specificity, but also faces issues like lengthy detection time and low sensitivity. This paper proposes a method by using novel SERS-active silver flower-like material to enhance the fluorescence signal generated by qPCR instrument, so as to shorten the detection time and improve the detection sensitivity. In this method, silver flower-like material is first prepared on substrates using a lyotropic liquid crystal soft template combined with electrodeposition growth. Then, an ultrasonic device is used to peel the active material into aqueous solution, and the peeling efficiency reaches 90%. Using rhodamine 6G (R6G) as the qPCR amplification detection molecule, the fluorescence signal g

SUBMITTER: Song C 

PROVIDER: S-EPMC11836446 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature