Guide to the structural characterization of protein aggregates and amyloid fibrils by CD spectroscopy.

ABSTRACT: Protein aggregation and amyloid formation are linked to numerous degenerative diseases, such as Alzheimer's or Parkinson's disease. Additionally, protein aggregation plays a crucial role in various biological processes, such as storage of molecules or cell signaling. Protein molecules can form a wide range of aggregates, from oligomers of different sizes to non-specific aggregates and highly ordered cross-β structured amyloid fibrils with diverse morphologies. Circular dichroism (CD) spectroscopy is a widely used technique to study protein structures providing detailed information at the secondary structure level, and is ideal to distinguish and characterize protein aggregates. Despite its potential, CD spectroscopy is often perceived as having limited application on protein aggregates due

SUBMITTER: Kardos J 

PROVIDER: S-EPMC11836901 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature