Integrated analysis reveals that EGR1 promotes epithelial IL33 production in T2 asthma.

ABSTRACT:

Background

Airway epithelial cells constitute the first line of defense against external noxious stimuli and play crucial roles in the release of epithelial inflammatory cytokines (IL33, IL25 and TSLP), initiating airway allergic inflammatory diseases such as asthma. IL33 plays critical physiological processes in T2-endotype asthma. However, the mechanisms by which allergen exposure triggers IL33 release from airway epithelial cells remain unclear.

Methods

Integrated bioinformatic analysis and transcriptional analysis of bulk RNA-seq and single cell RNA-seq (scRNA-seq) data were used to identify core genes and determine the internal gene network associated with IL33. The expression of EGR1 was subsequently analyzed in vitro in the BEAS-2B cell line and in vivo in a house dus

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC11837401 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature