Assembly and performance of a cholera RDT prototype that detects both <i>Vibrio cholerae</i> and associated bacteriophage as a proxy for pathogen detection.

ABSTRACT: Cholera rapid diagnostic tests (RDTs) are vulnerable to virulent bacteriophage predation. We hypothesized that an enhanced cholera RDT that detects the common virulent bacteriophage ICP1 might serve as a proxy for pathogen detection. We previously developed a monoclonal antibody (mAb) to the ICP1 major capsid protein. Our objective was to design and assemble a first-of-its-kind RDT that detects both a bacterial pathogen (Vibrio cholerae) and associated virulent bacteriophage (ICP1). Candidate mAbs were expanded to increase design options and evaluated by immunological assays (ELISA; western blot). A subset of mAbs were selected for gold conjugation and printing on the RDT. The detection limit of the prototype RDTs was determined in diarrheal stools with the addition of ICP1. Three m

SUBMITTER: Sayeed MA 

PROVIDER: S-EPMC11837499 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature