CRISPR-guided base editor enables efficient and multiplex genome editing in bacterial cellulose-producing <i>Komagataeibacter</i> species.

ABSTRACT: Bacterial cellulose (BC) is an extracellular polysaccharide produced by bacteria that has wide applications in the food industry, tissue engineering, and battery manufacturing. Genome editing of BC-producing Komagataeibacter species is expected to optimize BC production and its properties. However, the available technology can target only one gene at a time and requires foreign DNA templates, which may present a regulatory hurdle for genetically modified organisms. In this study, we developed a clustered regularly interspaced short palindromic repeats (CRISPR)-guided base editing method for Komagataeibacter species using Cas9 nickase and cytidine deaminase. Without foreign DNA templates, C-to-T conversions were performed within an 8 bp editing window with 90% efficiency. Doub

SUBMITTER: Xin B 

PROVIDER: S-EPMC11837512 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature